代写毕业论文
5) In the FLT3 TKD pcr products, the gel shows that the undigested FLT3 TKD gives a band of around 290 bp size. Upon digestion with Eco RV, the WT will give two bands at 187 and 91 bp. If two bands are not seen, then the recognition site in the gene may be mutated. In all the test samples, two bands are seen at the sizes corresponding to that of WT, showing that no mutation has taken place in the Eco RV recognition site.
6) Generally the AML patients are known to show an average level of mutation when their karyotyping is done. Around 45% of them show normal karyotyping results. It may be because of the heterogenecity which is seen in the disease. One of the allele remains unmutated i.e. in normal form while the other allele gets mutated. This generally gives normal karyotyping results. Hence better form of diagnostic methods like PCR is needed to be used. Expression of FLT3 Tyr Kinase receptor is generally seen in progenitor haematopoietic cells. If it’s expressed in the committed or differentiated cells, then the patients is prone to AML. Mutation can occur in form of either internal tandem duplication or in the Tyr kinase domain. The former lead to autophosphorelation of the receptors and thus more formation of AML cells will take place. Different studies have shown that these mutations are prime cause for AML induction and AML cell proliferation. PCR analysis of the specific sequences can help us identify AML. (Bacher, Ulrike, and et al. 2007, p.g.2527)
代写毕业论文
5）在FLT3 TKD PCR产物的凝胶，表明未消化的FLT3 TKD给出了一个带约290 bp的大小。在与生态RV消化，重量会在187和91 bp的两条带。如果两个波段都没有见过的，然后在基因识别部位可以突变。在所有的测试样品，两个频带被视为在对应的重量大小，显示没有突变在生态RV识别位点发生。
6）的AML患者通常被称为显示突变的平均水平时，他们进行了染色体核型分析。其中45%的显示正常核型分析结果。这可能是因为该heterogenecity是疾病。一个等位基因仍然未即正常形式，其他等位基因发生突变。这通常给正常核型分析结果。因此，更好的诊断方法，如PCR需要使用。FLT3酪氨酸激酶受体的表达，一般是在祖造血细胞出现。如果它在提交或分化的细胞中表达，并易于AML患者。突变可以在内部串联重复的形式或在酪氨酸激酶结构域发生。前者导致的受体，从而形成更多的autophosphorelation AML细胞会发生。不同的研究表明，这些突变的主要原因为AML诱导AML细胞的增殖。的特定序列的PCR分析可以帮助我们确定急性髓细胞白血病。（巴彻等人，健身，和。2007，p.g.2527）
